EDNA shufing can also be performed using multiple related gones. Which of the fo

Question

EDNA shufing can also be performed using multiple related gones. Which of the following have been impro in this manner a. Insulin b. Erythropoietin c. Beta-lactamase d. Zinc fingers 36 Which of the following is NOT an example of a combinatorial protein library a. DNA coding for various protein domains recombined in a variety of ways b. Exons and alternate exons being recombined in a variety of ways C. Random PCR products from a d. PCR products all over a genome are recombined into pieces of DNA and cloned corresponding to protein motifs are randomly combined. 37. The basis of many biomaterials NOT a result of this engineering is protein engineering with elastin-like polypeptides. Which of the following is a. The ability of a doctor to inject liquids that then form gels b. Zinc finger domains are sued to turn on elastin-like polypeptide genes c. Peptides can promote cell migration and adherence d. Movement and growth of vascular smooth muscle cells 38 Which of the following is NOT a domain associated with protein-protein binding a. Beta-sandwich b. Three-helix bundle c. Zinc finger motif d. Disulfide-bonded scaffolds e. Small scaffolds 39. In order to produce recombinant proteins in mammalian cells, single cells must be isolated. Match each of the technologies designed to isolate cell lines with its best description. Flow cytometry Gel microdrop technology Laser-enabled analysis and processing b. Cells containing recombinant protein and reporter protein. The reporter is measured as the c. Cells are immobilized, fluorescent antibody is added, the brightest cell is identified and the rest d. LacZ gene fusions and X-gal are used to measure activity cells are sorted. are killed Cells containing recombinant protein are immobilized in a biotinylated agarose, the production of recombinant protein is captured and assessed by biotin-avidin reactions. Fluorescence is measured e. 40. With respect to HPLC, match each detector system below with its best description of that type. Refractive index_ Ultraviolet_ Fluorescence a. Use of UV light to determine when a molecule passes through. b. Use light to determine when a molecule passes through and refracts light. c Uses light to determine when molecules are absorbing light and emitting a different wavelength. d. Detects radioactively labeled molecules. 41. Match the following each of the following proteases to its catalytic site protease class Elastase Papain

Explanation / Answer

Question 35- The DNA shuffling method includes random mutations to make DNA library. This method provides a fast way to produce library. The DNA shuffling is very useful for Pharmaceutical industry and Vaccine formation. Therefore, the correct answer is Beta-Lactamase, which is an antibiotic.

The 4th option (d) is the correct answer.

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