7. Which of the following is the best description of Proteomic Pattern diagnosti
ID: 257983 • Letter: 7
Question
7. Which of the following is the best description of Proteomic Pattern diagnostics? a. Finding protein marker for disease and then examining changes from normal to disease via mass spectrometry Obtaining blood serum samples of healthy patients and patients with disease, followed by mass spectrometry. The diseased b. n analyzing selective portions of data to find differences between normal and c Use of radioisotopes such as H, C, and NIS to label proteins being produced by normal cells and diseased cells. Then using mass spectrometry All of the above. d. e. None of the above. 8. Which of the following changes in a protein CANNOT be detected using mass spectrometry? a Cleavage with an endoprotease. b. Cleavage with an exoprotease c. Phosphorylation. d. Glycosylation. e. All of the above CAN be detected with mass spectrometry 9 His 6 tags are used in purifying proteins because: a. Histidine binds to Ni so a Nit column can be used to bind the protein of interest b. Six histidines can be added to either the amino-terminal end or the carboxy-terminal end of the protein without disrupting overall structure. c. The protein can be removed from the column using histidine or imidazole. d. All of the above. e. Some of the above 10. Biopanning involves all of the following EXCEPT a. Screening a series of surface proteins for ability to bind a target such as an antibody. b. A sequence of 6 histidine at the beginning of each peptide. c. Use of a phage display library. d. The bacteriophage M13 e. All of the above are involved in biopanning em is used to study protein-protein interactions. Which of the following is true of a two- hybrid system. a. Proteins are fused to either a DNA binding domain (bait) or an activation domain (prey). When proteins interact, a reporter gene is turned on. b. The sy c. The DNA binding domain and the activation domain are from the maltose binding protein operon. d. All of the above. e. None of the above. stem is designed to work using E. coli but does not work in other organisms 12. The significance of metabolomics is a. b. It consists of all the small molecules in the biological system. It indicates the flow in the complex network system with in the cell. c It can be studied with cells grown in Ci glucose or c" glucose followed by chromatography or NMR. All of the above. d.Explanation / Answer
Q. 7: I feel the option A is correct because the mass spectroscopic analysis is completely based upon the m/z ratio i.e mass/charge ration. In this the indentification of any protein associated with the diseased content would give a clear picture of the diagnosis and development of the disease hence it should be accurate proteomic pattern diagnosis.
Q.8- The option e is correct because mass spectrometery (MS) is one of most coprehensive technique which can analyse the complicated protein structure either in pre or post traslation modifications in the protein structure however the protein oxidation some time become little difficult to analyze by MS but combination of hydrogen/deuterium exchange? with MS gives the analysis of any of the complicated protein structure.
Q.9: The option d is correct because poly histidine or histidine 6 is new invented protein motif developed by Roche having the capabilities to bind protein at N or C terminal. In addition it easily binds with nikel or cobolt which makes purification faster in presence of any dye such as imidazole?.
Q.10: The option b is incorrect. As in the process of bioplanning first the specific antibodies are identified which is having ability to affinity with the M13 by using the phase display library. Later the affinity coaded antibodies analysed using ELISA technique.
Q.11: The option a is correct. The term two hybrid refers "bait" as first one which infuse protein to DNA and "prey" is second one in which fusion of DNA transcriptional associated domain. This techniques is helpful to identify expession system in yeast and helpful to develop cDNA library.
Q.12: The option c is correct. As metabolomics is the new science of study which helps us to diagnose a disease at specific point of time and lenght of the disease. Hence it require a series of analysis which includes targetted chromatography follwed by MS-GC (mass spectrometry-gas chormatograpy) or NMR. Later data of sequence or protein generated and analysed by using library based information avaliable in the bioinformatics tools.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.