You are working with a new molecule that you think may be an effective antibioti

Question

You are working with a new molecule that you think may be an effective antibiotic. Below is some experimental data you have collected. You inoculated a series of liquid cultures with different concentrations of drug to a starting OD of 0.050. The next day, you read the optical density of those cultures. Then, you take cells from that culture and plate them on solid media without drug. 6. OD of a liquid culture grown with drug 0.845 0.793 0.826 0.067 0.053 0.064 0.066 Number of colonies on a plate without drug 6.4 x 108 6.2 x 108 6.3 x 108 3.5x 107 3.6 x 107 3.4 x 107 5 Drug concentration No drug 1 ug/mL 2 ug/mL 4 ug/mL 8 ug/mL 16 ug/mL 32 ug/mL What is the MIC of your molecule? a. b. What is the MBC of your molecule? c. Is your molecule bactericidal or bacteriostatic? Why?

Explanation / Answer

minimum bactericidal concentration (MBC) is the lowest concentration of an antimicrobial agent required to kill a particular bacterium.

minimum inhibitory concentration (MIC) is the lowest concentration of a chemical which prevents visible growth of a bacterium.

the MIC of the molecule is 4 ug/ml. and

MBC is 32 ug/ml.

this molecule is bacteriostatic in nature. because 8 fold lower concentration is needed to hamper the growth of the bacteria in a culture.

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