Match the best term to the definition or example that is given below (Not all te

Question

Match the best term to the definition or example that is given below (Not all terms will need be used. Some terms may be used more than once.) phenotype of?ofZ'ITOC Z+ phenotype of l+ O+ Z' / ? OC Z+ regulatory molecule must be present at a DNA site (such as a promoter) so that A. negative control B. noninducible C. constitutive D. temporal control E. repressible F effector molecule G. inducible transcritpion occurs small molecules that bind to a regulatory protein such as a repressor a mutation that reverses the effect of another mutation at a different site post-translational control of a biosynthetic pathway phenotype with respect to B-galactosidase for the partial diploid o+zir ocz H. repressor attenuator region J. positive control K. suppressor mutation L. feedback inhibition M.operator during the lytic cycle of A bacteriophage, genes concerned with DNA replication are transcribed before genes specifying head proteins, which are transcribed before tail proteins phenotype with respect to the synthesis of tryptophan enzymes in the trp operon that is trpRt oC st where trpR codes for repressor and S is a structural gene R codes for phenotype of trpR+ Oo+ S+ in the presence of tryptophan (again, trp repressor and S is a structural gene) type of operons that respond to catabolite repression (the glucose effect) regulatory molecule that binds to the operator region of a DNA molecule

Explanation / Answer

1. Constituitive.   In Oc mutation of lac operon, there are changes in the DNA sequence of the operator that impair the binding of the lac repressor. As a result, the repressor will never bind to the operator even in the absence of inducer. Since the cell has Z+ genotype also, there will be constituitive expression of the lac genes.

2. Constituitive. There will be constituitive expression of lac genes because there is no repressor synthesized or the repressor is mutated and will not bind to the operator.

3. Operator. operator is a segment of DNA near the promoter to which a transcription factor binds to regulate gene expression.

4. effector molecule. An effector molecule is usually a small molecule that selectively binds to a protein and regulates its biological activity.

5. Supressor mutation. A suppressor mutation is a second mutation that reverses the phenotypic effects of an already existing mutation. It restores the phenotype seen prior to the original background mutation.

6. Feedback inhibition. Feedback inhibition regulates the activity of already existing proteins. It occurs at the post transcriptional levels. It is the phenomenon where the product of a pathway is used as an input to control the behavior of the pathway, oftentimes limiting the production of more product.

7. Inducible. I-/Oc/Z-, this ic constituitive but since lac Z genes are mutated, it will non iducible. But I+/O+/Z+ is normal inducuble phenotype.

8. Temporal control. Temporal control of gene expression in lambda bacteriophage is accomplished in a cascade, in which one gene product produced at each stage is required to stimulate gene expression in the next stage.

9. Constituitive. In Oc mutation there are changes in the DNA sequence of the operator that impair the binding of the trp repressor. As a result, the repressor will never bind to the operator even if it is in abundance. So constituitive expression of tryptophan will be there.

10. Repressible. This condition is the normal trp operon in cell and is repressible in presence trp. This means that there will be no synthesis of the structural in presence of trp encoded by trpR gene.

11. Positive control. the CAP-cAMP (catabolite repression) system is an example of positive control, because expression of the operon requires the presence of an activating signal which in this case is the interaction of the CAP-cAMP complex with the CAP region.

12. Repressor. The repressor binds tightly to the operator preventing the transcription of genes.

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