Thymine (T) Thymidine Thymidine monophosphate (TMP) Uracil (U) Uridine Uridine m

Question

Thymine (T) Thymidine Thymidine monophosphate (TMP) Uracil (U) Uridine Uridine monophosphate (UMP) The Standard Genetic Code First Position (S end) Second Position Position (3' end) UUU Phe UCU Ser UAU Tyr UGU Cys UUC Phe UCC Ser UAC Tyr UGC Cys UUA Leu UCA Ser UAA Stop UGA Stop A UUG LeuUCG Ser UAG Stop U CUU Leu CCU Pro CAU His CGU ArgU CUC LeuCCC Pro CAC His CGC Arg CUA Leu CCA Pro CAA Gln CGA Arg A CUG Leu CCG Pro CAG Gln CGG Arg AUU lle ACU Thr AAU AsnAGU Ser AUC lle ACC Thr AAC Asn AGC Ser AUA lle ACA Thr AAA Lys AGA Arg A AUG Met ACG Thr AAG Lys AGG Arg UGG Trp G GUU Val GCU Ala GAU Asp GGU GlyU GUC Val GCC Ala GAC Asp GGC Gly GUA ValGCA Ala GAA Glu GGA Gly GUG Val GCG Ala GAG Glu GGG Gly 1. Using the codon chart in your text (inside Back Cover) translate the following message in three reading frames assuming that the ribosome has already started translating the message upstream: NNNCUGUAUAGAGCCUGAUCGGCGGNNN 2. a. What is the anticodon sequence in the tRNA of the charged tRNA that would bind to last readable codon in the message below (N maybe in your answer)? 5' NG CUGUGCG CGCN3' b. What amino acid would be present on this tRNA? This exercise uses an online 3D structure analysis software. Go to RCSB PDto download structures [over 10,000 files!!] At the home site enter 5EMQ in the search window upper

Explanation / Answer

5' NNN CUG UAU AGA GCC UGA UCG GCG GNN N 3'

1) frame 1 - X L Y R A & S A X

5' N NNC UGU AUA GAG CCU GAU CGG CGG NNN 3'

frame 2 - X C I E P D R R X

5' NN NCU GUA UAG AGC CUG AUC GGC GGN NN 3'

frame 3 - X V @ S L I G G

here @ and & are stop sequences so after that chain will terminate.

2) mRNA sequence is 5' N GCU GUG CGC GCN 3'

tRNA sequence is 3' N CGA CAC GCG CGN 5'

amino acid sequence will be- R H A R

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