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1) the segmentts could join in the following order: 2) The segments could also j

ID: 259506 • Letter: 1

Question

1) the segmentts could join in the following order:

2) The segments could also join in this order:

Please reinforce your knowledge of recombinant DNA technology by visualizing how the following DNA fragments might "stick" together. These pieces can join in the following sequence: segment 1, seg- ment 2, segment 3, segment 4, segment 6, and segment 5. Demonstrate your understanding of recom- binant DNA by listing two additional sequences in which the segments could join. The segments could join in the following order er:

Explanation / Answer

As, in the case of recombinant technology, proper orientation of sticky ends play an important role. So, the segments could join in the following order:

3 followed by 2 followed by 1 followed by 4 followed by 6 followed by 5 followed by 2 followed by 3 followed by 4 followed by 1 followed by 5 and lastly 6.

The segments could also join in this order:

3 followed by 2 followed by 4 followed by 1 followed by 5 followed by 5 followed by 2 followed by 3 followed by 4 followed by 1 followed by 5 and lastly 6.