Biot 120 Exam 3 Spring 2018 4.List 4 types of chemical contamination (Spt) 4. To
ID: 259999 • Letter: B
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Biot 120 Exam 3 Spring 2018 4.List 4 types of chemical contamination (Spt) 4. To be useful, cell line must be le Niaiel should also be grown in the absence of lt that might mask contamination (Spt) N?adle/uoaA!..et Ants kises 5. Kara A. isolates cells from her own bone marrow at Montgomery College Hospital. She isolates a clone from fraction 3, clone D5. Using ICLAC guideline, what would the suggested cell line name be? (5pt) MCH-B-12-D5 List 4 potential non-sterile contacts that can occur while attempting to perform your cell culture aseptically. (5pt) 2 7. You are designing an experiment to test for endotoxin using Rabbit Fever Assays. List what you would use for the positive control and negative control. What would the positive result in the assay be? (5pt) PC= NC- Positive Result= You are designing an experiment to test for virus using an infectivity assay (think Mink Test). List what you would use for the positive control and negative control. What would the positive result in the assay look like (what you see)? (5pt) NC- Positive Result-Explanation / Answer
7.
PC = known endotoxin such as "WHO-LPS 94/580"
NC = pyrogen-free saline
Positive result = the compound is pyrogen ice if individual temperature rise above 0.5°C, temperature rise in group above 1.4°C (for group of 3 rabbits).
8.
PC = Well characterized virulent virus such as "Epstein-Barr virus"
NC = sterile (virus-free) serum
Positive result = infected cells.
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