Horizontal transfer of DNA commonly occurs among prokaryotes. You observe DNA ca

Question

Horizontal transfer of DNA commonly occurs among prokaryotes. You observe DNA can readily transfer between two species of bacteria; Species A and Species B. To delineate which mechanism of DNA transfer is responsible you set up the following experiment. . Control 1: Species A . Sample 1: Species A + genomic DNA of Species B Sample 2: Species A+ DNA nuclease . Sample 3: Species A + genomic DNA of Species B+ DNA nuclease Sample 4: Species A + Species B After this experiment you only observe DNA transfer occurring in Sample 4, and no other samples or your negative control. The DNA nuclease used will degrade naked, extracellular DNA. Which of the following DNA transfer mechanisms may be responsible for transfer of DNA from Species B to Species A? Transfection Transformation Conjugal transfer Translocation None of thesc mcchanisms

Explanation / Answer

Conjugal transfer

Since DNA transfer is observed only in sample 4 where two species are involved. Conjugal transfer is the most suitable mechanism where both cells come on direct contact forming a bridge to facilitate the horizontal DNA transfer.

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