Practice Question Chronic granulomatous disease is a condition where phaygocytes
ID: 261156 • Letter: P
Question
Practice Question Chronic granulomatous disease is a condition where phaygocytes are unable to make reactive oxygen species in order to kill some bacteria and fungi resulting in chronic infections. This disease can be caused by mutations in a number of genes, but the most common gene involved is the gp91PHOX gene. We had 3 patients that were diagnosed with this disease, and we knevw that gp91PHOX was the cause of their disease. We performed Northern Blots on the patients as well as a control sample that did not have this disease. We got the following data (Lane 1- control; Lane 2- Patient 1; Lane 3- Patient 2; Lane 4 Patient 3): A. (4 pts) Predict what is wrong with this gene in Patient 1 and tell me why you think this? B. (4 pts) Predict what is wrong with this gene in Patient 2 and tell me why you think this? C.(4 pts) Predict what is wrong with this gene in Patient 3 and tell me why you think this? D. (2 pts) Why did we need to run the control sample to answer this question? E. (2 pts) What did we isolate from the patients to do this test (DNA, RNA, protein)? F. (2 pts) What tissue type in the body did we isolate the answer from E.Explanation / Answer
A. Since the band migrates slower in patient 1, there appears to be an insertion in the genomic locus of the patient 1 gp91PHOX gene.
B. Since the band migrates at the same pace as that of the control in patient 2, it appears to be a substitution mutation in the genomic locus of the patient 2 gp91PHOX gene.
C. Since the band migrates faster in patient 3, there appears to be a deletion in the genomic locus of the patient 3 gp91PHOX gene.
D. The control sample shows exactly where the WT mRNA migrates. It is used for comparing mutant samples.
E. It is a northern blot. It is done on RNA samples.
F. The given gene is expressed in phagocytes. Phagocytes are abundantly present in the blood. So, RNA could be isolated from the blood.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.