6. You are performing a mating experiment with the following strains. Donor: Rec

Question

6. You are performing a mating experiment with the following strains. Donor: Recipient: F' lac hisA'/AlacAhis Lac+ Hist amps, F. ?1ac hisA1 Bla"-) Lac, His, ampr The recipient strain contains a point mutation (hisA1) in the hisA gene and is therefore auxotrophic for histidine biosynthesis; it also contains a chromosomal copy of the beta lactamase gene. You are given the following glucose-minimal salts plates: GM - glucose minimal salts plate GMA - glucose minimal salts plate containing ampicillin GMH - glucose minimal salts plate containing histidine Indicate which plate you would use to grow the donor and recipient and which plate you would use to select for the exconjugant. The donor: The recipient: The exconjugant

Explanation / Answer

The donor: GM plate ( because donor has ampicillin sensitive gene , so cannot grow on GMA plate. it has His gene , so no need to grow on GMH plate).

The recepient: GMH plate ( the recepient strain is auxotrophic for histidine, thus it cannot grow on minimal media, hence needed to be suplemented with histidine).

The exconjugant: Gm plate ( exconjugants are partners in conjugation which exchanged genetic material . recepient bacterial cell that has been seperated from the donor after conjugation contains genes of Donors DNA)

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