3. You are attempting to acquire synaptic current recordings from crayfish muscl

Question

3. You are attempting to acquire synaptic current recordings from crayfish muscle onto a computerized data acquisition system using a loose patch electrode and a current amplifier. Your signal is being fed into an oscilloscope, and the output from the rear of the oscilloscope is being fed, in turn, to the computerized data acquisition system. The recording on the computer screen shows a flat line centered at 0 mV, as shown by the solid line in the diagram below. r+5.000 V 0 V -5.000 V 1.000 s 2.000 s 3.000 s List IN ORDER all the steps that should be taken to determine why the synaptic currents are not visible on the computer screen.

Explanation / Answer

Steps involved to in loose patch clamp technique are:

1. Move the pipette slowly towards the cell such that the resistance between the cell and electrode is increased few times more than the electrode alone.

2. Care should be taken not to bring pipette too close to the cell, as it may become difficult to remove the pipette and the cell may get damaged.

3. Also, the cell membrane should not be pierced at all.

4. The cell membrane should be intact to allow free passage of ions through the gap.

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