ral glucose tolerance test with 3 patien uation for standard calibration graph d
ID: 261734 • Letter: R
Question
ral glucose tolerance test with 3 patien uation for standard calibration graph data obtained using an enzyme-based glucose assa y = 3.2726x-0.0009 Table 1:Absorbance data from an enzyme-based glucose assay for diluted patient blood samples (dilution: 75 blood +825 u saline). Ben 0.360 0.698 0.579 Fasting value 1 hour value 2 hour value Ashley 0.402 0.825 0.673 Conno 0.284 0.564 0.325 Absorbance (A 500 nm) OGTT blood samples for each patient were taken before the test (fasting value), at 1 hour post- glucose load (1 hour value) and 2 hours post-glucose load (2 hour value) IMPORTANT NOTE: A l patient blood samples were diluted (75 ??blood + 825 ?L saline) and then assayed for glucose using the same enzyme-based glucose assay as used for the standard glucose calibration graph. Table 2:Patient blood glucose concentrations (mmol/L) from oral glucose toleranc sts as determined using an enzyme-based glucose assay Using the standard calibration equation to determine the glucose concentrations (mmoVL) for all the patient samples. Remember you will need to correct for dilutions and Patient Blood Glucose Concentrations (mmol/L) Fasting value 1 hour value 2 hour value Ben Connor results nterpretation of .Normal fasting plasma glucose (measured at the start of the OGTT before the glucose load is administered) should be below 6.1 mmol/L and fasting levels repeatedly at or above 7.0 mmol/L are diagnostic of diabetes A 2 hour OGTT glucose level between 7.8 mmol/L ? 11.1 mmol/L indicates "impaired glucose . Fasting levels between 6.1 mmoVL and 7.0 mmol/L are borderline ("impaired fasting glycemia"), .A 2 hour OGTT glucose level below 7.8 mmolL is normal, whereas higher glucose levels indicate · tolerance" .A 2 hour OGTT glucose level above 11.1 mmoUL confirms a diagnosis of diabetesExplanation / Answer
Answer:
Based on the given raw data:
These values are converted to concentration values (in mg/ml) using the linear regression equation:
Y = 3.2726*X - 0.0009
Where Y = Absorbance value and X = Concentration of glucose in mg/ml
For example for Absorbance value of 0.402, the concentration of glucose can be determined from standard curve equation as below:
X (mg/ml) = (Y + 0.0009) / 3.2726 = (0.402 + 0.0009) / 3.2726 = 0.1231
Since the sample was diluted in the beginning (75 ul + 825ul saline = 900ul), fold dilution = 900/75 = 12 x (12 fold), therefore each concentration value was multiplied with fold dilution value to get the actual concentration in sample in mg/ml
Next, all these values are converted from mg/ml to mMol/L by dividing with Molecular mass of glucose (180.156 g/mol) and multiplying with 1000.
Absorbance data Ashley Ben Connor Fasting value 0.402 0.36 0.284 1 hour value 0.825 0.698 0.564 2 hour value 0.673 0.579 0.325Related Questions
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