( Template strand ) 5’ - AGA GGC GAC ACA CTT TGA CCG ACC GTA AGG - 3’ A. What is

Question

(Template strand)

5’ - AGA GGC GAC ACA CTT TGA CCG ACC GTA AGG - 3’

A. What is the mRNA sequence produced when this gene is transcribed? Indicate 5’ and 3’ ends. Hint: note that this is the template strand- see Fig. 12.11, p. 245 in your text.

B. What is the protein sequence produced when this mRNA is translated? For this particular example, translate the sequence without the start and stop codons.

D. If the sequence above were the coding strand, what would be the mRNA sequence?

UAU UAC UGC UUA UCA UAA Stop UGA Stop A UUG CUU cuC CUA CUG AUU AUCÌlle ACC! AAC? AGC| |C AUA AUG ACGAAGAGG GUU GUC GUA GUG Leu UCGUAG Stop UGG Trp G CCU CAU CGU CAC CCA CCG ACU CGA Gin CAG AAU AGU Ser ACA GCU GAU GGU GCC GAC Ala Gly GGA GCG GAG

Explanation / Answer

Given template strand sequence: 5’- AGAGGCGACACACTTTGACCGACCGTAAGG-3’

mRNA sequence: 5'-UCUCCGCUGUGUGAAACUGGCUGGCAGUCC-3'

Protein sequence: Ser-Pro-Leu-Cys-Glu-Thr-Gly-Trp-Gln-Ser

Arrow mark is missing.

If the first C is changed G; GGC to GGG = CCG to CCC = Pro to Pro

If the second C is changed G; GAC to GAG = CUG to CUC = Leu to Leu

If the third C is changed G; ACA to AGA = UGU to UCA = Cys to Ser

Non-template strand sequence: If the given sequence were to be the coding strand,

3'-TCTCCGCTGTGTGAAACTGGCTGGCAGTCC-5'

mRNA sequence: 5'AGAGGCGACACACUUUGACCGACCGUAAGG-3'

Protein sequence: Arg-Gly-Asp-Thr-Leustop-Pro-Thr-Val-Arg

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