EXTRA CREDIT. (3 Points) A generalized transducing phage is used to transfer gen

Question

EXTRA CREDIT. (3 Points) A generalized transducing phage is used to transfer genes from astad hist+)met()phe(+) donor strain of E coli to a hist-)metphe(-) recipient strain. When t on a medium containing methion tants selected ine are tested for growth on other media, many more colonies are found on media supplemented only with histidine than on media supplemented only with methionine transductants survive on a basal , No I medium. What is the correct gene order and which 2 genes are most closely inked?

Explanation / Answer

his+met+phe+ is crossed with his-met-phe-. High degree of co-transduction indicates linked genes.Here there were more colonies found on media supplemented only with histidine.There are lesser number of colonies with metionine plates. Lesser transductants indicate double cross overs. This means the genes are closely linked.The genes His and Phe are closely linked.

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