Q3. Your lab has recently identified a small molecule present in e-cigarette vap
ID: 261941 • Letter: Q
Question
Q3.
Your lab has recently identified a small molecule present in e-cigarette vapor called TX32. You suspect it may be toxic and want to test its effects on mitochondrial function and membrane potential more specifically.
You prepare four dishes with cells. You treat the cells with three different doses of TX32 and leave one untreated for comparison (Figure A).
The following day, you add a small amount of TMR for 1 hour (Figure B) and use a fluorescence microscope to measure the TMR signal in each dish (Figure C).
Figure A. Dishes of treated and untreated cells.
Figure B. Addition of TMR to each cell culture dish.
Figure C. Example of TMR signal in a cell.
Membrane Potential Experiment
1 point possible (graded)
You observe a significant decrease in TMR signal in TX32-treated cells that varies by dose (Figure A). How might TX32 affect membrane potential?
Figure A. TMR signal in treated and untreated cells.
Select ONE option:
1. Directly inhibits ETC proton pumping activity like rotenone or antimycin.
2. Interfere with glucose metabolism and/or TCA cycle and thereby reduce NADH and FADH2 supply to ETC
3. Increase the proton leak through the membrane like FCCP.
4. All of the above.
Untreated TX32 Low Dose TX32 Medium Dose TX32 High DoseExplanation / Answer
Correct answer is 4. All of the above.TMR signal is affected by antimycin, FCCP and TCA cycle inhibitors.
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