7. (a) A patient suffering from tuberculosis is being treated using a standard c

Question

7. (a) A patient suffering from tuberculosis is being treated using a standard course of medication. To see how effective the treatment is, a fresh sputum sample is sent to the laboratory. What molecular technique(s) would you use to determine (4 marks) treatment effectiveness and why? (b) There is a codon deleted in the cystic fibrosis gene in patients suffering from this genetic disease. Apart from DNA sequencing, describe the molecular procedures (4 marks) that you would use to identify this small deletion? (c) Suggest molecular techniques that could be used for detecting large chromosomal (2 marks) deletions. 8. and STR in DNA profiling. (b) Compare and contrast DNA profiling in civil and criminal cases. (a) What is satellite DNA in the human genome? Briefly discuss the use of VNTR (6 marks) (4 marks)

Explanation / Answer

7 A) sputum smear would be subjected to microscopy so as to identify the efficiency of the tuberculosis treatment regimen. Sputum smear would be prepared so as to identify the thick mucous which is the characteristic feature of tuberculosis, is indicative of the clearance of the mycobacterium.

B)

mutation detection methods are as follows:

C) Microarray: DNA or cDNA are taken from the normal cells, followed by labelling with a fluorescent dye which are combined as well as hybridised for forming a microarray.

Restriction fragment length polymorphism: this techniques Is carried out such that there different samples of the homologous DNA are analyses.

Related Questions

Navigate

• Browse (All)
• Browse 7
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.