lc Desl answer. Answer 14 of 15 questions 1. Which of the following sequences is
ID: 262578 • Letter: L
Question
lc Desl answer. Answer 14 of 15 questions 1. Which of the following sequences is most likely to be a restriction sequence? a. GC b. ATA AGAG c. CGATCG d. GGAACCTT e. AACCTAGGTT 2. How does site-directed mutagenesis change targeted sequences? a. via PCR using oligonucleotide primers that include changes of interest b. via the Dicer protein (RNA interference) c. via the CRISPR-Cas9 system d. via positional cloning e. via dideoxynucleotide sequencing 3. How many different gametes can be produced by an organism with genotype AABBccDdEeFFgg? a, 2 b. 4 c. 8 d. 16 e. 32 4. Refer to the Manhattan plot shown below, which depicts results of a genome-wide associati study on Crohn's disease. What can you conclude? Crohn's disease 15 1 10 5 a. Four genes toward the end of chromosome 2 are associated with the disease. b. Crohn's disease is at least partly X-linked. c. More than one gene associated with the disease is located on chromosome 5. d. Dozens of genes are associated with the disease. e. Crohn's disease is not a polygenic trait.Explanation / Answer
The most likely answers are C.and E., as both are palindromic sequences.
C. CGATCG
GCATGC
E. AACCTAGGTT
TTGGATCCAA
Both these sequences , when read in both directions, are palindromic.
Restriction sequences or restrictions sites are palindromic sequences, which are cut by restriction enzymes, at particular sites between two nucleotides.
The cuts so generated can form blunt ends or sticky ends.
Each restriction enzyme identifies a novel restriction sequence.
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