1) Which of the following processes is dependent on endonuclease activity? Proof
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Question
1) Which of the following processes is dependent on endonuclease activity?
Proofreading
Mismatch repair
Excision repair
Apoptosis
Mismatch repair, excision repair, and apoptosis
2) What problem associated with DNA replication is solved by the formation of Okazaki fragments?
The two strands of DNA are held together by millions of hydrogen bonds, making separation extremely difficult.
DNA must polymerize in the 5' to 3' direction on a template.
DNA polymerization requires an existing chain onto which to add new dNTPs.
DNA replication begins at multiple origins of replication in eukaryotes.
3) What would you observe in a population of cells with a mutation in Primase that caused it to think every T on the template strand was a C?
Primase would incorporate G instead of A into the primer, and the mutations would be passed down to the next generation in both strands of DNA.
Primase would incorporate G instead of A into the primer, and these mutations would be passed down to the next generation but only in one strand of the DNA.
Primase would incorporate G instead of A into the primer, but none of the mutations would be passed down to the next generation.
Primase would still incorporate the correct nucleotide because how it reads the template is irrelevant.
4) What kind of enzyme catalyzes polymerization of DNA?
Primase
Endonuclease
Polymerase
Helicase
5) Shown below is a diagram of a eukaryotic replication bubble with only the template shown. Where on the diagram is the leading strand? (Examine the template DNA very carefully before answering this question!)
A
B
C
D
Explanation / Answer
1) Which of the following processes is dependent on endonuclease activity? Proof
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