12) The figure below is from Maniak et. al. 1995. icty were fed fluorescently la
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12) The figure below is from Maniak et. al. 1995. icty were fed fluorescently labeled yeast in an assay similar to the ones we did in class. The Dicty cells The paper presents the results from an experiment where wild were treated with the drug Cytochalasin A, which inhibits actin polymerization. If you were going to repeat this experiment using beads but only had enough cells for three flasks which conditions would you chose. Justify your choices both in terms of the experimental design and the cell biology. Maniak at al. used a longer time scale than we did in class. Are there advantages to extend the experiment beyond 15 minutes. (15 points) 2 60 40 20 10 30 60 90 time (min Figure 6. Effect of Cytochalasin A on Phagocytosis of Yeast Particles by Suspended and Shaken D discoideum Cells Numbers on the right of the curves indicate micromolar concentrations of cytochalasin A in the cell suspension. Wild-type cells had been pretreated for 30 min with the drug. Control cells (0) received only the solvent, 0.5% (v/v) of dimethyl sulfoxide. Fluorescence intensities on the ordinate reflect numbers of internalized TRITC-labeled yeast part cles.Explanation / Answer
12) The figure below is from Maniak et. al. 1995. icty were fed fluorescently la
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