Design an operon: Design a hypothtical bacterial operon that can metabolize iron

Question

Design an operon: Design a hypothtical bacterial operon that can metabolize iron, aluminum, or steel.

It will exclusively turn on an iron metabolizing pathway when all three (iron, aluminum, and steel) are available

it will turn on aluminum and steel metabolizing pathways when no iron is available

Mutant Y always produces all metabolic genes no matter which metabolites are available, Mutant X turns on only a steel metabolizing pathway when all three metabolites are available, and Mutant Z fails to turn on any metabolizing pathway independent of iron, aluminum or steel presence. This mutation, however, is not in the catalytic pathway components. ONLY draw pictures, and include a glossary.

Explanation / Answer

Normal Metal (Met) Operon-

where,

MetI= inducer gene; MetP= Promoter; MetO= operator; MetFe= gene producing iron metabolising enzyme; MetAl= gene producing Alumunium metabolising enzyme; MetS= gene producing steel metabolising enzyme.

Mutants-

MetIs = super-repressor mutant results in no transcription of any gene at all, repressor unable to bind inducer

MetOc= constitutive mutant, the operator gets altered due to which repressor cannot bind it resulting in expression of the gene in absence or presence of an inducer e.g. metal here.

Mutant Y operon-always produces all metabolic genes no matter which metabolites are available

Mutant X turns on only a steel metabolizing pathway when all three metabolites are available

Mutant Z fails to turn on any metabolizing pathway independent of iron, aluminum or steel presence

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