You are interested in gene X and analyzed gene X by PCR (with enzyme digestion),

Question

You are interested in gene X and analyzed gene X by PCR (with enzyme digestion), a Northern blot (i.e., looking at all transcripts corresponding to gene X), a Western blot (i.e., looking at all proteins corresponding to gene X.

M (bp) T 1 M (bp WT 12 3 M (kD T 1 23 PCR gel RNA blot protein blot A. What, if any, gene X mutations are there in unknowns 1,2, and 3? What type of possible mutation is present (i.e., frameshift, nonsense, deletion, etc.) B. Which would you expect phenotypically for unknowns 1,2, and 3 (i.e., would you expect the unknowns to be wild type, show a phenotype, etc.)? 2) You are following four traits (a, b, c, and d). The following crosses were performed Cross 1: a b female x cd male F1 progeny: all wild type Cross 2: F1 female x F1 male F2 progeny: 1600 wild type (total 6400) 400 b d 800 a 400 a b 1200 ? 400 dc 800 b A. Describe the inheritance pattern for each trait. Be as complete as possible. B. Which, if any, genes are linked? Can you provide a map distance between the genes?

Explanation / Answer

1)A) in this question , if you observe PCR gel, restriction enzyme digestion produce two frament in wild type, which similiar in unknown 2 means unknown 2 has nonsense mutation , in this mutation codon that code for amino acid change to stop codon but this change occur at protein level. Therefore, in northern blot there is no change but protein are truncated thats why western blot show protein of different molecular weight.

Unknown 3 show one fragment which means point mutation occur in gene X lead to missing restriction site means single fragment generated and thats why northern blot and western blot both show fragment of different size as compare to wild type.

Unknown 1also has deletion but it doesnt affect restriction site. So there is two fragment , one of large size and another is of same size of wild type.

B) unknown 2 and 3 has different phenotype than wild type due to mutation. Unknown 1produce protein fragment of same size as wild type and has a similiar phenotype like wild type

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