How will you determine where and when the mouse genes are expressed? Select the

Question

How will you determine where and when the mouse genes are expressed?

Select the four correct statements.

Expression of Hoxa7 and Hoxb7 could be studied by PCR to identify tissues containing the genes. Expression of Hoxa7 and Hoxb7 could be studied by using antibodies to detect the proteins. Multiple samples using animals at different stages of development would be needed to assess the temporal pattern of gene expression. Expression of Hoxa7 and Hoxb7 could be studied by translational gene fusions of GFP or lacZ to Hoxa7 and Hoxb. Expression of Hoxa7 and Hoxb7 could be studied by in situ hybridization to identify tissues containing the mRNAs. Multiple samples using animals of different species would be needed to assess the species-specific pattern of gene expression.

Explanation / Answer

Gene expression effectively means transcription of DNA to mRNA. Once mRNA expression is done, protein will automatically be synthesized. Therefore both mRNA and proteins corresponding to respective genes of interest are indicative of the gene expression. To study both the temporal (WHEN) and spatial (WHERE) patterns of gene expression, it is important to collect tissues at different stages of development and also to identify the tissues expressing the desired genes. With respect to this, we can refer to the following four statesments:

1) Multiple samples using animals at different stages o development would be needed to assess the temporal pattern of gene expression.

2) Expression of Hoxa7 and Hoxb7 could be studied by in situ hybridization to identify tissues containing the mRNAs.

3) Expression of Hoxa7 and Hoxb7 could be studied by translational gene fusions of GFP or lacZ to Hoxa7 and Hoxb7.

4)Expression of Hoxa7 and HOxb7 could be studied by using antibodies to detect the proteins.

The remaining two options do not hold correct, as PCR will amplify the gene from each and every tissue, as the genes are present in the DNA of each cell. It is only the gene expression that is regulated (ectopic expression) in a tissue specific manner. Besides multiple samples from different species will not address the questions asked (WHEN and WHERE?)

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