Northern Blot ( the sizes of the bands and a description of the blot is given) .

Question

Northern Blot ( the sizes of the bands and a description of the blot is given) . zoom in screen to see blot.

Northern Blot with ssRNA marker and random, non-specific probe binding. Lane 2 (biotinylated ssRNA ladder). Lane 4 (extracted mRNA)-liver tissue, lane 6 (tRNA liver tissue, lane 8 (tRNA)spleen tissue.

thick bands at 7000nt, 5000nt, and 3000nt.

Half of gel it there is random probe binding (seen as darker areas) in a diagonal line.

What is the significance of these results, Please explain in detail to understand the significance the results in the northern blot. If not going to be specific, then please allow someone else to answer. 1-3 sentences is not enough

Explanation / Answer

According to the image, it can be clearly seen that Northern blot analysis of liver extract mRNA and tRNA was made in this experiment. However, either of the extracts failed to provide any positive bands and only non-specific binding mediated patches were observed on lower side of the gel. This observation suggests following:

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