enzyme A enzyme B Figure 3: Metabolic Pathway Refer to the metabolic pathway ill
ID: 266499 • Letter: E
Question
enzyme A enzyme B Figure 3: Metabolic Pathway Refer to the metabolic pathway illustrated in Figure 2. Substance C is required for the cell's growth. In your experiment, you irradiate the cell's DNA to cause mutations to the enzyme genes. Sure enough, the cell doesn't grow after radiation unless supplemented. Now you need to figure out which gene was mutated. Use this information to answer the questions below. You supplement your mutated cell culture with substances A, B and C. Do the cells grow? Why or why not? 5. You supplement your mutated cell culture with substance B only. The cells grow! Is enzyme B's gene mutated? How do you know? 6. 7. You supplement your mutated cell culture with substance A only. The cells do not grow! Is enzyme A's gene mutated? How do you know?Explanation / Answer
5. On providing A, B and C.
If A , B C all are given together then the cell will grow. Reason: Even if the enzymes to manufacture them are missing still A , B and C are present for the cell growth.
6. If only B is provided and the cell grows, it proves that gene B is not mutated. As gene B is not mutated so it is able to manufacture C for proper growth of the cell.
7. On adding A, The cell does not grow. Either gene for enzyme A or Gene for enzyme B could be mutated that’s why final conversion to C is not happening and cell will not grow
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