1) You are interested in gene X and analyzed gene X by PCR (with enzyme digestio
ID: 266571 • Letter: 1
Question
1) You are interested in gene X and analyzed gene X by PCR (with enzyme digestion), a Northern blot (i.e., looking at all transcripts corresponding to gene X), a Western blot (i.e., looking at all proteins corresponding to gene X.
A. What, if any, gene X mutations are there in unknowns 1, 2, and 3? What type of possible mutation is present (i.e., frameshift, nonsense, deletion, etc.)
B. Which would you expect phenotypically for unknowns 1, 2, and 3 (i.e., would you expect the unknowns to be wild type, show a phenotype, etc.)?
M (bp) WT 1 2 3 M (bp) WT 1 23 M (kD) WT 1 23 PCR gel RNA blot protein blotExplanation / Answer
1.
Lane 1- deletion since losing the size on the gel
Lane 2- frame shift mutation by addition of some nucleotide
Lane 3- nonsense mutation by chain termination since RNA and protein blot is losing the size.
2. 1 can behave as WT since it has same protein and RNA as per blot.
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