Transforming growth factor (TGF-B1) has been shown to upregulated (increased exp

Question

Transforming growth factor (TGF-B1) has been shown to upregulated (increased expression) in various forms of cancer. Madine-Darby Canine Kidney (MDCK) cells were cultured in the absence or presence of TGF-?1. In addition, TGF-?1 stimulated cells were treated with drug A'. Both adherens junction formation as well as cell motility was assessed. Control TGF-B1 TGF-B1Drug 'A Control TGF-B1 TGF-1 Drug 'A Control TGF-1 TGF-B1 Drug 'A' ? (A) Cultured MDCK kidney cells were treated with control, transforming growth factor-B1 (TGF-B1), or TGF-B1+Drug 'A" for 24 hours. I performed by labeling cells for E-cadherin (green) and nuclei (red). (B) Phase- contrast images of cultured cells treated with similar conditions as (A). Cel morphology indicates cells that cluster as a sheet (control) or disperse throughout the culture dish (TGF-B1). (C) Scratch assay of cells grown in the presence of either control or TGF-B1. Phase-contrast images were taken immediately following the formation of the cell-free zone (0 hour) and 24 hours following scratch formation (24 hour). was What might be the effect of drug 'A' on cell dispersion when added to TGF-B1 treated cells? Use the data to support your conclusions. a. b. What might be the effect of drug 'A' on cell motility when added to TGF- B1 treated cells? c. What effect might TGF B1 have on RhoA activity? Use the data to support your conclusions.

Explanation / Answer

Answer A.

the cells treated with TGF-beta will show less dispersion in the culture medium when treated by drug A. This is evident from results of immunoflourescence images. Drug A enables proper E-Cathedrin formation.

Answer B.

The cell motility is reduced due to reformation of E-Cathedrin after treatment with drug A.

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