Chapter 16 Loss of Function 6 points total enzyme A enzyme B Figure 3: Metabolic
ID: 266703 • Letter: C
Question
Chapter 16 Loss of Function 6 points total enzyme A enzyme B Figure 3: Metabolic Pathway Refer to the metabolic pathway illustrated in Figure 2. Substance C is required for the cell's growth. In your experiment, you irradiate the cell's DNA to cause mutations to the enzyme genes. Sure enough, the cell doesn't grow after radiation unless supplemented. Now you need to figure out which gene was mutated. Use this information to answer the questions below. 2 points each 14. You supplement your mutated cell culture with substances A, B and C. Do the cells grow? Why or why not? 15. You supplement your mutated cell culture with substance B only. The cells grow! Is enzyme B's gene mutated? How do you know? 16. You supplement your mutated cell culture with substance A only. The cells do not grow! Is enzyme A's gene mutated? How do you know?Explanation / Answer
Given pathway,
A -----> B -----> C
The given pathway produces C as the end product. So, if cells are supplemented with C, the pathway becomes redundant.
14. If the mutant cells are supplemented with A, B, and C, they will grow. This is because the end product of the pathway is present in the culture so there is no need of the enzymatic production of C by the pathway.
15. If the mutant cells grow in the presence of B in the culture medium, it shows that there is no mutation in the enzyme B. It also suggests that the mutation is in the enzyme A.
16. If the mutant cells do not grow in the presence of A alone, it means that the mutation could be in enzyme A or enzyme B.
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