Adding two restriction enzymes in a restriction digest: provides stability to th

Question

Adding two restriction enzymes in a restriction digest:

provides stability to the ligated DNA strands

ensures the correct direction of DNA being inserted in the plasmid.

is rarely a good idea, since it typically leads to a drop in transformation efficiency.

helps the plasmid DNA strands ligate to each other

provides stability to the ligated DNA strands

ensures the correct direction of DNA being inserted in the plasmid.

is rarely a good idea, since it typically leads to a drop in transformation efficiency.

helps the plasmid DNA strands ligate to each other

Explanation / Answer

Option 4 is the correct answer.

Adding two restriction enzymes in a restriction digest helps the plasmid DNA strands ligate to each other. Addition of two restriction enzymes ensures the creation of compatible ends along with sticky end overhangs that confer ease of ligation between the DNA insert and vector. The success of a DNA recombination methods is dependent upon restriction enzyme digestion to generate compatible ends capable of being ligated together.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.