To know which RNA polymerase has transcribed a certain gene Add a toxin to diffe

Question

To know which RNA polymerase has transcribed a certain gene

Add a toxin to differentially inhibit RNA polymerases and quantify isolated mRNA

Tag RNA polymerase with a radiolabeled signal and monitor the product using EMSA

Isolate mRNA and reverse transcribe it to cDNA

Quantify the DNA using southern blot and then amplify using PCR

Add a toxin to differentially inhibit RNA polymerases and quantify isolated mRNA

Tag RNA polymerase with a radiolabeled signal and monitor the product using EMSA

Isolate mRNA and reverse transcribe it to cDNA

Quantify the DNA using southern blot and then amplify using PCR

Explanation / Answer

B. Tag RNA Polymerase with a radiolabeled signal and monitor the product using EMSA.

EMSA can deduce the interactions between molecules so, radiolabeled enzyme can be found associated with the sequence it is going to transcribed.

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