help with this genetic problem You have a mutant E.coli with a lac operon (lacMu

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help with this genetic problem

You have a mutant E.coli with a lac operon (lacMuT) which is not responding to lactose control You create an F episomal plasmid with the wild-type lac operon (lacW) and transform it into your mutant E. coli Genotype Mutant (lac) Mutant (lac Merizygote (lacMUT+ lacMUT)No Merizygote (lalacYes Lactose Added? Beta-galactosidase activity 100% 100% 100% 200% MUT Yes MUT MUT Is the mutation in the lacl trans-factor or the lacO cis-element? Describe the likely mutation and explain why you conclude that. 8.50 x 11.00 in

Explanation / Answer

Explanation:- The mutation most probably would be in lacO cis-element because in merizygote the where repressor from wild lac operon is normal but fue to mutation in operator region it is not able to bind the operator thus cannot prevent lac operon expression thus giving 100% beta galactosidase activity.When lactose is added to the medium repressor molecules bind to lactose or allolactose and become inactive which further increases lac operon expression to 200% ( beta galactosidase activity).

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