In the indole test the activity of what enzyme is tested for? If there is a posi
ID: 268089 • Letter: I
Question
In the indole test the activity of what enzyme is tested for?
If there is a positive indole test, what amino acid must have been present?
What is tested for in the Methyl Red Test?
What enzyme is present in a methyl red test?
If a pure culture has a positive methyl red test and butanediol production was also tested for, would you expect a positive or negative result? Explain
What is tested for in the Voges-Proskauer Test?
Name the three media which are stabbed when they are inoculated
Why does the agar turns blue in a positive Citrate test?
Name two bacteria which give a positive results on the Citrate test
If a Proteus culture is inoculated into a urea broth and a red purple color is observed after a 20 minute incubation at 37^C, is this a positive or a negative test?
What enzyme must be present for a positive urea reaction?
E.coli, staphylococcus aureus, and Enterococcus faecalis display what type of oxygen requirements?
Name an anaerobic organism that was discussed in lab this semester
Describe Brownian motion
The oil immersion objective provides what total magnification?
Which part of the microscope has the purpose of focusing the radiation from light source onto the specimen
What type of microscope is used in this lab?
What does parfocal mean?
Name a structure that is extremely heat resistant What is the solution used to clean the slides?
How many times is a smear from liquid culture heat fixed?
How many minutes is a slide with a smear allowed to air dry?
How many times is the loop flamed when a quadrant streak is performed?
Name the only eucaryote that was encountered in this lab
Name the bacterium that produces a red pigment
What is the name of the red pigment?
What is the name of the test method for antibiotic sensitivity?
What is the test medium for the antibiotic sensitivity testing?
What is measured in the antibiotic sensitivity testing?
What medium is used to test for hemolysis?
What is the equation for determining the doubling time?
Consider the following data from a growth curve experiment: When the first sample was taken, the cell concentration was 5 x 10^5 cells/ml. When the second sample was taken 90 minutes later, the cell concentration was 4 x 10^6 cells/ml. What is the doubling time of the population? Do not use equation or calculator to answer this equation
What test uses Kovac’s reagent?
Describe the negative test in the previous example
A positive methyl red test demonstrates what?
Methyl red test orange + or - yellow + or -
What test contains pH indicator bromothymol blue?
What is the original color of the agar for the previous test?
Bacteria may be distinguished on the basis of surface differences (i.e polyssccharides on the surfecase of the bacterial cell). What are these differences called?
Explanation / Answer
1. determine the presence of the enzyme tryptophanase.
2. tryptophane
3. bacteria producing stable acids by mechanisms of mixed acid fermentation of glucose
4. glycolytic enzymes
5. Bacteria that have the butanediol fermentation pathway are positive for the VP test.
6. ACETOIN
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