tell me the blue one RI 81%. 9:31 K Question Anonymous Asked about 9 h ith a bri
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tell me the blue one
RI 81%. 9:31 K Question Anonymous Asked about 9 h ith a bright water (Figure ID) A). We use the se micelle as well encapsulating different hydrophilic reagents is around use DOTAP can 140 nm, and the polydispersity index (PDI) is smaller than thus ensuring an 0.25 by dynamic light scattering DLS) Table1). RNV the water core loaded with different hydrophilic reagents shows marginal and cholesterol) differences in size and PDI The surface charges of the RNV LGA shell in the encapsulating different reagents can be influenced by the The measured hydrodynamic diameter of the RNV achieve a long contents Briefly, the positively charged DOTAP may diffuse to the outer lipid layer, resulting in the positive surface charge s, such as calcein, of RNV when encapsulating calcein and rhodamine B. How- core of RNV by ever, for fabrication of RNV encapsulating siRNA, the apment efficiency negatively charged SiRNA will interact with the DOTAP e. of calcein (or thus limiting the diffusion of DOTAP and resulting in the pment efficiency negative surface charge of RNV (Supporting Information). tometer and the The entrapment efficiencies of three hydrophilic reagents into s S2 and S3). To the RNV are almost 90%(Table 1), approximately 1.5 times e RNV SiRNA, of free Table Size (d), zeta potential (), polydispersity index (PDl) and entrapment RNV is efficiency (EE) of RNVs loaded with different reagents cation). RNV/lreagent Z-AVE, (RNV ount of RN A] (the RNVjcalcein] of free RNVDox/siMDR1] 136.413.81414.23.25 0.2220.017 91.23 +4.51 SiRNA I RNV Information. ?? 196] d Inm 130.6?14.10 49.82.815 112+0.35 0.1320.018 90.66+0.2 V[rhodamine] 14.1?1.760.221?0.027 89.68 ± 1.47 (al Z-AVE-mean particle size. [b Details can be found in the Supporting ??2?9????2?????????? Table 1: Size (d), zeta potential (5), polydispersity index (PDI) and entrapment efficiency (EE) of RNVs loaded with different reagents. RNV/[reagent] Z-AVE, d [nm EE [96] RNVIrhodamine 130.614.10 14.11.76 0.2210.027 89.681.47 RNVIcalcein] 149.8+2.815 1.20.35 0.1320.018 90.660.27Explanation / Answer
In this experiment hydrodynamic diameter of various RNV encapsulating different hydrophilic reagents are measured. It ia found to be approx. 140nm. The different reagents encapsulated affects the surface charge of RNV. Like diffusion of DOTAP to outer lipid layer results in RNV's positive surface charge when encapsulating calcein and rhodamine B. But in case siRNA, due to its negative charge it interacts with DOTAP. So, it's diffusion is limited and imparts negative surface charge to RNV.
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