Answer the questions below regarding the following DNA microarray experiment. Th
ID: 268640 • Letter: A
Question
Answer the questions below regarding the following DNA microarray experiment. The purpose of the microarray was to screen a genomic library for genes that were expressed in normal cells (green), cancer cells (red), or both from ovarian tissue. 3 4 9 What is immobilized on the DNA chip? a. b. What was the source of the DNA that is labeled with red fluorescent dye? What causes this DNA to bind to the microarray? C. What was the source of the DNA that is labeled with green fluorescent dye? What causes this DNA to bind to the microarray? d. What causes sectors of the array to fluoresce yellow?Explanation / Answer
Please find the answers below:
Answer a: DNA microarray is conducted to investigate the presence of specific target sequences on the host DNA. In order to do so, previously known sequences of DNA in the form of short nucleotides are immobilized on a sterile and inert surface such as silica probe. Thus, short oligonucleotides of known sequences are immobilized on the surface.
Answer b: The red-spots on the microarry correspond to cancerous cells hence cancerous genes in the cells. Hence, the red-spots correspond to cancerous genes in the cells.
Answer c: The green-spots on the microarray correspond to normal cells in the breast tissue, hence correspond to non-cancerous genes.
Answer d: The presence of yellow spot demonstrates the presence of cancerous genes in the normal cells i.e. either onset of cancerous genes or established expression of cancerous genes in breat tissue cells.
Thus, the intensity of different spots can be measured as a quantitative estimation of total gene expression in the breast tissue under normal and cancerous conditions.
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