lt sequence but may make llar cloning (examples: EcoAL, EcoBl, EcoD TY PE ? : re

Question

lt sequence but may make llar cloning (examples: EcoAL, EcoBl, EcoD TY PE ? : recognizes and cleaves specific sequences length),; (examples: EcoRI, BamH TYPE 1I1 cimiln- , HindI, Xbal); usec cells volume Dilution factor cells_volume + trypan_blue_volume total number of_cells nonviable cells(blue) 8, 1 00 %viability total_number_of_cells The U937 cells were treated as shown in the table below for 48 hours. Determine the percent viability for each group of the cells as well as the total number of cells, 1. The wortmannin weighed 1 mg and it was dissolved in 233 ul of dimethylsulfoxide (DMSO). The molecular weight of wortmannin is 428.43 g/mol. What was the concentration of the stock solution? The stock solution above was diluted to other stock solutions to achieve concentrations that were 1000 times higher than the intended final concentrations to be added to the cells. This way, l ?1 of the appropriate stock solution was added 1 ml ofcell culture medium to achieve a 1000 fold dilution. This avoids adding excessive amounts of the solvent to the cells, which can obscure the effect of the drug. 2. Triciribine was purchased from Sigma-Aldrich (St. Louis, MO) and it weighed 5 mg. The molecular weight is 320.30. The desired concentration in the culture medium was 5 ?M. Explain how the stock solution/solutions should have been prepared and added to 2 ml of cell culture medium. Total number of cells %viability Treatment Control (medium) Control (DMSO) Plot the data on graph using a spreadsheet program, such as Excel with the dose of the drug on the X-axis and cell viabilities on the Y-axis. 3. .2

Explanation / Answer

Answer:

Answering 1 and 2 part.. 3rd part needs data generated in lab.

1)

2)

Related Questions

Navigate

• Browse (All)
• Browse L
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.