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You have isolated 2 different strains of bacteria (bacteria A and bacteria B) bo

ID: 26923 • Letter: Y

Question

You have isolated 2 different strains of bacteria (bacteria A and bacteria B) both of which uptake and metabolize the monosaccharide galactose to various degrees. You suspect that the galactose is being taken up by a sodium (Na+)-coupled galactose transporter. To test the possibility that the 2 different bacteria are utilizing a Na+-coupled transporter, you isolated and grew each bacteria in the appropriate growth medium. Galactose uptake was measured in the presence and absence of sodium. Below are the results of this experiment. Please interpret the results. Are bacteria A and bacteria B both likely to contain Na+-coupled galactose transporters? Why or why not?

Explanation / Answer

Bacteria A likely contains a Na+ coupled galactose transporter, while bacteria B probably doesn't. The reason for this is that bacteria A saw a nearly 50% reduction in growth in the absence of NaCl, while bacteria B saw no change in growth in the presence of NaCl compared to the absence of NaCl.

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