The Staphylococcl: Isolation and identification (continued) 2 Microscopy Provide

Question

The Staphylococcl: Isolation and identification (continued) 2 Microscopy Provide drawings here of your various isolates as seen under oil i NOSE FOMITE 3. Record of Culture and Test Results GROWTH FERMENTATION ALPHA- COAGULATION ONASE ZONE NOVOBIOCIN OF CLEARING (S/R) HEMOLYSIS ON BAP (n) ON MSA OF OF (n) |MANNITOL («HI | PLASMA (-) Nose Fomite If API See Exercise 43. Nose Fomite Unknown control Staph miniaturized multitest strips are available, confirm your conclusions by testing each isolate. B. Short-Answer Questions 1. Describe the selective and differential properties of mannitol salt agar (MSA) for the isolation and iden- tification of staphylococci. 2. Describe the differential property of blood agar for the isolation and identification of staphylococci. 368

Explanation / Answer

1. MSA act as a selective medium as it contain high amount of salt. Hence, all bacteria could not grow in that media except staphylococcus. MSA act as differential media because it contain sugar mannitol.which they used as fermentation substrate and convert phenol red to yellow.

2. Staphylococcus aureus produce a toxin that perform the lysis of the blood. Other species of staphylococcus does not do that. Hence, it is used as differentiatial media.

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