5.) DNAsel Footprinting You perform 4 DNAsel footprinting experiments. All the e

Question

5.) DNAsel Footprinting You perform 4 DNAsel footprinting experiments. All the experiments contain DNA 1. Experiments 2,3, and 4 also contain Protein A which binds DNA 1 in the indicated position. DNA 1 is labeled by radio labels on the indicated ends. Arrows show positions that DNAsel can cut. Draw the banding pattern you expect to see in the 4 different experiments in the box on the right. (30 points) Experiment 20 40 60 80 Experiment 1: DNA 60 40 20 Experiment 2 DNA1 Experiment 3 DNA1 Experiment 4 DNA 1

Explanation / Answer

DNAse1 do not cut at the point where protein is bound to DNA sequence thus for experiment 2, 3 and 4 at 60kda marker bands will be ,missed rest all the bands will be present on gel.

+ means band present

blank means no band present(footprint)

marker 1 2 3 4 80 + + + + 60 + + + + 40 + + + + 20 + + + +

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