1) What is the enzymatic function of restriction enzymes? A) To add new nucleoti
ID: 270220 • Letter: 1
Question
1) What is the enzymatic function of restriction enzymes?
A) To add new nucleotides to the growing strand of DNA.
B) To join nucleotides during replication.
C) To join nucleotides during transcription.
D) To cleave nucleic acids at specific sites.
E) To repair breaks in sugar-phosphate backbones.
2) Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?
A) restriction enzymes.
B) gene cloning.
C) DNA ligase.
D) gel electrophoresis.
E) reverse transcriptase.
Explanation / Answer
1) What is the enzymatic function of restriction enzymes?
Restriction enzymes are the types of endonucleases which cleave the DNA at speicific site called restriction sites. As these enzymes cleaves the DNA those are called molecular scissors.
So, here the answer is D) To cleave nucleic acids at specific sites.
2) Which of the following seals the sticky ends of restriction fragments to make recombinant DNA?
The answer is C) DNA ligase.
As it binds the DNA framents with nucleophilic attack these are reffered as molecular glues.
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