Questions 1. Give two factors that influence the size of an individual\'s DNA fr
ID: 270394 • Letter: Q
Question
Questions 1. Give two factors that influence the size of an individual's DNA fragments after it Ih acted on by a restriction enzyme 2. The masses (sizes) of the four nucleotides in DNA are different-look them up. Why do those different masses not affect results of an agarose gel electrophoresis experiment? That is, suppose we had a 400 base-pair fragment composed of all A on one strand all T on the other strand. Why would that fragment travel the nearly same distance as a G-C 400 base- pair strand?Explanation / Answer
The factors that affect the size of DNA when acted upon by the restriction enzyme include
1. Frequency of restriction sites: more the frequency of these sites, lesser will be the size of the fragments and vice versa
2. Activity of enzymes: more the active enzyme, more will it produce digestion products
3. Presence of enzymes like nucleases that cut the DNA non-specifically
4. Presence of inhibitors and other solvents like chloroform, ethanol etc
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.