(1) Answer the three questions for each of the following transcription factors:

Question

(1) Answer the three questions for each of the following transcription factors:

LacR

TrpR

CAP

GAL4

GR (glucocorticoid receptor)

(a) What is the small molecule that controls its’ activity?

(b) In the presence of this molecule, is the protein on the DNA or off the DNA?

(c) What is the consequence for transcription?

(2) Fill out the table with the words ON or OFF:

- lactose:

lac repressor ON or OFF the DNA

- lactose:

lacZ ON or OFF

+ lactose:

lac repressor ON or OFF the DNA

+ lactose:

lacZ ON or OFF

I+ O+ Z+

I- O+ Z+

I+ Oc Z+

I+ O+ Z+ / Oc

I+ O+ Z+ / I-

I- O+ Z+ / I+

I+ Oc Z+ / O+

(3) Answer the three questions for each of the following enzymes:

Swi/Snf

HAT

HDAC

DNMT

(a) Describe the activity of each of the enzymes.

(b) What is the consequence of this enzyme activity?

(c) How does this enzyme know what genes to act on?

- lactose:

lac repressor ON or OFF the DNA

- lactose:

lacZ ON or OFF

+ lactose:

lac repressor ON or OFF the DNA

+ lactose:

lacZ ON or OFF

I+ O+ Z+

I- O+ Z+

I+ Oc Z+

I+ O+ Z+ / Oc

I+ O+ Z+ / I-

I- O+ Z+ / I+

I+ Oc Z+ / O+

Explanation / Answer

2)

- lactose:lac repressor ON or OFF the DNA

- lactose:

lacZ ON or OFF

+ lactose:lac repressor ON or OFF the DNA

+ lactose: lacZ ON or OFF

3) a) Activity of each enzymes -

b)

c)

- lactose:lac repressor ON or OFF the DNA

- lactose:

lacZ ON or OFF

+ lactose:lac repressor ON or OFF the DNA

+ lactose: lacZ ON or OFF

I+ O+ Z+ On Off Off On I- O+ Z+ Off ON OFF ON I+ Oc Z+ ON ON Off ON I+ O+ Z+ / Oc ON ON OFF ON I+ O+ Z+ / I- ON ON OFF ON I- O+ Z+ / I+ ON ON OFF ON I+ Oc Z+ / O+ ON On OFF ON

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