You obtain a DNA sample from your patient and send it to a lab for sequencing. T
ID: 270757 • Letter: Y
Question
You obtain a DNA sample from your patient and send it to a lab for sequencing. They sequence of pro-insulin. Compare the sequence of the normal gene to that of the patients. a) What difference have you found? b) Is this a TRANSVERSION or a TRANSITION? c) What type of mutation is this REGULATORY, or STRUCTURAL (in the coding region)? d) Is the mutation frameshift, missense, conservative, non-conservative, nonsense or none of the above? Be sure to check with your amino acid codon chart 1 agccctccag gacaggctgc atcagaagag gccatcaage agatcactgt cettctgcca 61 tggccctgtg gatgcgcctc ctgcccctgc tggcgctgct ggccctctgg ggacctgace 121 cagccgcage ctttgtgaac caacacctgt tcggctcaca cctggtggaa gctctctacc 181 tagtgtgegg ggaacgaggc ttettctaca cacccaagac ccgccgggag gcagaggacc 241 tgcaggtggg gcaggtggag ctgggcgggg gccctggtgc aggcagcctg cagcccttgg 301 ccctggaggg gtccctgcag aagcgtggca ttgtggaaca atgetgtacc agcatctgct 361 ccetctacca gctggagaac tactgcaact agacgcagcc cgcaggcagc cccacacccg 421 ccgcctcctg caccgagaga gatggaataa agcccttgaa ccagcaaaa Ala.1ysPro.The.Tyr.Phe.Phe.Gly A chain stan Arz NH, NH NH NH chain start To add a new twist, the protein Insulin has quaternary structure but it has only one gene. When it is in the ER, Pro- insulin is cleaved into two polypeptides (A and B) and put back together to give the protein shown above. III. Given the diagram of insulin above, what do you think is the problem with the protein from your patient? IV. When you received the DNA sequence for the patient, you discovered that only one of the alleles had the Please use your answer to the mutation you found in part II mutation, the other allele was wild type. Having two different alleles for the same gene is called V. Your patient is diabetic, while your patient's sister is wildtype and does not have diabetes. Individuals with two mutant alleles cannot survive. This is called ? dominance
Explanation / Answer
Answer a) It will be smaller than normal.
Answer b) Transversion
Answer c)Structural
Answer d)conservative
Answer III) now the insulin will be cleaved in 2 parts.
Answer V) true
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