a. DNA is antiparallel. b. DNA is a linear molecule. c. DNA is negatively charge
ID: 271017 • Letter: A
Question
a. DNA is antiparallel. b. DNA is a linear molecule. c. DNA is negatively charged d. DNA is wound around histones. 77. Which of the following statements about the genetic code are most accurate? Select all that apply a. The promoter sets the reading frame. b. The genetic code is generally overlapping. c. An initiation codon sets the reading frame. d. Three initiation codons set the three possible reading frames. 78-80. During the bacterial transformation experiment using E. coli and pGLo plasmids, you used the following agar plates: Plate A: pGLO, LB Plate B: pGLO, LB/amp Plate C: +pGLO, LB/amp Plate D: pGLO, LB/amp/arabinose 78. Which of the following is NOT a feature of a plasmid? a. It is a circular DNA molecule. b. It is a part of a bacterial chromosome c. It is a supercoiled small molecule. d. It is naturally found in some bacterial cells. 79. Which of the following is a difference between the results seen on plate B and plate C? a. Plate B contains living, untransformed cells; plate C contains living,transformed cells b. Plate C contains living, untransformed cells; plate B contains living, transformed cells. c. Plate B contains dead, untransformed cells; plate C contains living, transformed cells. d. Plate C contains dead, untransformed cells; plate B contains living, transformed cells. 80. On which plate(s) do you expect to see green E.cof colonies under UV light? a. Plate A b. Plate C c. Plate D d. Plates C&D; 81-83. We used PCR and agarose gel electrophoresis to detect human Alu insertion dimorphism. In the photograph, identify the correct genotype for each lane as a. b. C. d. It cannot be determined Genotype for DNA in lane 1 is Genotype for DNA in lane 3 is 83. Genotype for DNA in lane 4 is 81. 82. 84. Which of the following is not true regarding cancer cells? Cancer cells divide rapidly and continuously. a.Explanation / Answer
79) Option c and Option d: Reading frame divides the sequence of nucleotides in a nucleic acid into set of consecutive, non-overlapping triplets. If the triplet codes for an amino acid, it is called as codon. The initiation codon (codon that codes for first amino acid of the protein) sets the reading frame on nucleic acid. Since the triplet contains three nucleotides, three different initiation codes will set three different reading frames on the same nucleic acid.
78) Option b: Plasmids are circular or super coiled, extra-chromosomal DNA molecules found naturally in the bacterial cells.
79) Option c: Plate B contains untransformed cells that have not taken up the pGLO vector and are dead because of inability to show resistance towards ampicilin present in the LB agar. However, plate C contains transformed cells which show resistance against the antibiotic ampicilin, owing to the vector, and are thus alive.
80) Option c: Plate D in which arabinose is added to LB agar in addition to antibiotic will grow E.coli colonies that will show green fluorescence under UV light. This is because in presence of arabinose, along with the arabinose metabolizing gene, green fluorescence protein (GFP) gene is expressed, the product of which express fluorescence under uv light.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.