3 What is the primary negative consequence of not spreading the inocuium evenly

Question

3 What is the primary negative consequence of not spreading the inocuium evenly over the agar surfacer 4 To get isolated colonies on a plate, only about 300 cells can be in the inoculum. What will bappen if the cel density of the inoculum significantly exceeds this mumberi Suppose you have two organisms in a mixture and Organism A is 1,000 times more abundant than Organism B. Will you (without counting on good luckl) be able to isolate Organism B using the spread plate techniue? Explain your answer

Explanation / Answer

3. The primary negative consequence of not spreading the inoculum evenly over the agar surface is that, they got clumped and not able to isolate with each other, so they are not countable.

4. So isolated colonies can not be obtained if the cell density of the inoculum significantly exceeds this number.

5.Suppose if two organisms in a mixture and Organism A is 1000 times more abundant that organism B. Then it is quite difficult to isolate organism B using the spread plate technique because it is Organism A is 1000 times more abundant that organism B.

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