if the answer refers to neither Chemical Fume hood Biological Safety Cabinet bot

Question

if the answer refers to neither

Chemical Fume hood

Biological Safety Cabinet

both

neither

Used to dispense hazardous chemicals

Used when working with patient samples

Equipped with HEPA filter

Equipped with UV light.

5. Purpose of the HEPA filter:

controls air flow rate

protects the sample from possible contamination external substances

sterilizes the air flowing through the cabinet

6.Purpose of the UV light:

provides additional light for working

protects the sample from possible contamination

sterilizes the cabinet at end of sampling

Centrifuge Parts (match the part in Column A with the one correct function in Column B)

Column A 1. Rheostat ______ 2. Bucket _______ 3. Trunnion ring _____ 4. Brake _______

Column B A. sets spinning time B. speed control C. tube holder D. holds bucket E. allow for faster slow down time ?

Types of Centrifuges (match the Centrifuge name in Column A with the one correct function in Column B)

Column A 5. Cytospine 6. Horizontal head 7. Microheamatocrit

Column B A. greater speed than angle head B. retains good cellular detail C. spins capillary tubes in hematology D. better separation of layers

8. An advantage of a horizontal head centrifuge is (may be more than 1 answer) a) gives best separation of layers b) spins faster than angle head due to less wind resistance c) holds more tubes

9. 8 tubes of whole blood were placed in a centrifuge. The speed was set for 2000 rpm for 10 minutes. The imbalance light came on as soon as the instrument started spinning. Choose from the list below all possible causes

a) rubber cushion missing from 1 or more buckets

b) tubes not placed opposite each other in buckets

c) blood volumes for opposite tubes not equal

d) tube sizes not all the same

10. If a method required spinning at an exact gravitational force, you must know the Relative Centrifugal Force for that centrifuge for a specific speed. The 2 things that will influence the RCF are: a) speed of rotation (RPM) b) size of the centrifuge (distance of tubes from motor shaft) c) nature of the samples to be spun RCF = 1.12R(RPM/1000)2, R= radius

11.State the steps to be followed if a tube of serum breaks in a centrifuge. ?

12. List all features that are not present for the centrifuge in this lab but will be present in centrifuges used in a biohazardous lab setting.

Explanation / Answer

When we expose our skin to UVB, it stimulates the production of vitamin D which our bodynneed how ever exposure of UVB can caise skin cancer

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