need help on finishing PCR Detection of GMOs and transformation of E. coli with

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need help on finishing PCR Detection of GMOs and transformation of E. coli with pGLO plasmid.

PCR Detection of GMO's: 1. What is a GMO? 2. How did we extract DNA from our foods? What was important about the water? And what specifically did the InstaGene matrix contain that helped protect against DNA degradation (and why)? Why did we also extract DNA from a non-GMO food? What does PCR stand for? Explain the process. What is important regarding the enzymes used? How did we make PCR specific for GM foods? What gene products did we test for? What were all relevant controls we utilized in our PCR? And what would it mean if any of the controls weren't as expected? 3. 4. 5. 6. Understand the relevance of gel electrophoresis for this application and how to interpret a gel result. Transformation of E coli with pGLO plasmid 1. What is transformation? Which scientist's experiment demonstrated this (and what was the experiment)? We 2. What does it mean for a cell to be competent? How can cells be made competent? Which method did we 3. The pGLO plasmid we used was recombinant DNA. What are all the relevant components of this plasmid were transforming E coli cells by giving it what characteristic? use? that was necessary for gaining successful results (transformation of E, coli)? 4. Media with ampicillin was used for what purpose? 5. Media with arabinose was used for what purpose? 6. What temperature was used to "heat-shock" the cells and why did we do this? 7. Why did we grow cells on LB media alone?

Explanation / Answer

Q1) GMO or genetically modified organism is a plant, animal, microorganism whose genetic material has been altered using genetic engineering techniques like PCR.  

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