1. In eukaryotes, DNA replication a) is catalyzed entirely by DNA polymerase III
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Question
1. In eukaryotes, DNA replication
a) is catalyzed entirely by DNA polymerase III
b) starts from multiple origins on each chromosome
c) does NOT require primer, only telomerase
d) does NOT require any remodeling of chromatin
2. When a 19kn fragment of DNA is cleaved with HindIII the fragments formed are 10 and 9 kb. AluI cleavage produces fragments of 17 and 2 kb. A double digest with HindIII and AluI produces fragments of 9,8 and 2 kb. Which of the following is true?
a) The AluI site is in the 9 kb Hind II fragment.
b) The AluI site is in the 10 kb HindIII fragment.
c) The HindIII site is in the 2kb AluI fragment.
d) AluI cleaves at 2 sites within the 19 kb fragment.
3. PCR uses:
a) one incubation temperature over 90 C
b) SI nuclease
c) electrophoresis
d) E.coli DNA polymerase
e) all of the above
4. In E.coli, DNA replication is:
a) conservative
b)unidirectional
c) accomplished by synthesis in the 5' to 3' direction
d) started from several origins
e) all the above are true
5. Telomerase
a) can be active in immortal cells
b) contains RNA
c) adds bases to the ends of chromosomes
d) all of the above
6. A Southern blot transfers ____ to a nitrocellulose membrane.
a) protein
b) RNA
c) DNA
7. In vertebrate genes, dispersed promoters:
a) start transcription at one specific base pair
b) are associated with genes that are transcribed constitutively
c) are rare or absent
d) all of the above
8. Enhancers:
a) are always located upstream from the TATA sequence
b) function in only one orientation
c) can be 2000 bp away from the TATA sequence
d) all of the above
9. In eukaryotes, transcription factors:
a) bind to the promoter before RNA polymerase binds
b) bind directly to RNA polymerase NOT to the DNA
c) bind to the operator NOT to the promoter
d) are NOT required for transcription
10. In eukaryotes, formation of mature mRNA may involve:
a) adding a poly A tail
b) adding a 5' cap
c) changing bases (RNA editing)
d) all of the above
11. Human gene therapy in the treatment of severe combined immunodeficiency (SCID) did NOT involve:
a) use of a modified retrovirus as vector
b) injecting the virus carrying the ADA gene directly into the patient
c) use of tissue culture
12. Histone Acetyl Transferase enzymes (HATs):
a) acetylate histone proteins to increase transcription
b) acetylate histone proteins to decrease transcription
c) deacetylate histone proteins to increase transcription
d) none of the above
13. In the lactose operon, the "z" gene codes for the enzyme B-galactosidase, "o" is the operator gene and "i" is the repressor gene. If an E.coli strain with the genotype i+o+z+/F'oc is grown on medium which contains no lactose or glucose, B-galactosidase ___ produced.
a) is not
b) is
14. cDNA's ____ introns
a) do not contain
b) do contain
Explanation / Answer
Ans 1 : b) Starts from multiple origins on each chromosome
The eukaryotic chromosomes have multiple origins , where the DNA replication can start. But for the DNA regulation , though multiple origins present , the replication initiates from each origin only once and also this occurs only once per cycle.
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