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con is a highly toxic, naturally occurring lectin that is produced in the seeds

ID: 272150 • Letter: C

Question

con is a highly toxic, naturally occurring lectin that is produced in the seeds of the castor oil plant. Ricin binds complex that Plx carbohydrates on the surface of eukaryotic cells. To prevent ricin toxicity you must identify the carbohydrate galac purple color develops when you add fucose, glucose and mannose in excess but there is no color when you add usin icin binds. To achieve this you perform an experiment on your cheek epithelial cells like you did in cell bio lab thang peroxidase inked to ricin. The four carbohydrates you test are fucose, glucose, mannose and galactose. You find ose. a) fucose Which sugar residue does Ricin normally bind to? glucos c) mannose d) galactose 100-fold dilution of the rabbit anti-human Nesprin antibody, at a starting concentration of 1.0 mg/ml would yeld which 2 of the following a) 1 mg/ml b) 0.001mg/ml c) 0.01 mg/ml d) 0.1 mg/ml concentrations? 9. During the immunohistochemistry lab you performed which of the following solutions dissolved the embedding compound and allowed antibody penetration? c) acla Yol Cleor

Explanation / Answer

Answer:

Which sugar residue does Ricin normally binds to?

8) A 100-fold dilution of the rabbit anti-human Nesprin antibody, at a starting concentration of 1mg/ml would yield which of the following concentrations?

9) During the immunohistochemistry lab you performed which of the following solutions dissolved the embedding compound and allowed antibody penetration?