Group Problems, Set 3 (Thurs. May 17) Name 1 Name 2 Name 3 Replication questions
ID: 272201 • Letter: G
Question
Group Problems, Set 3 (Thurs. May 17) Name 1 Name 2 Name 3 Replication questions, continued 1) Among the following, which enzyme creates the MOST phosphodiester bonds during replication in E coll? DNA ligse) DNA polymerase DNA polymerase I pria 2) Which enzyme creates the FEWEST pbosphodiester bonds during replication? )DNA ligaseb) DNA polymerase ) DNA polymerase II1 d) primase 5) Which of the folowing can both create and break (bydrolyze) phosphodiester (PDE) bonds during d)DNA pol 1 e) DNA pol m ?) telomerase b)topoisomerase/gyrasec) primase 0 DNA ligase g) helicase 4) Which of the following necessarily creates as many PDE bonds as it breaks? (circle ALL that apply) ?) telomerase b)topoisomerase/gyrasec) primase d)DNA pol l e) DNA pol m 0 DNA ligase g)helicase 5) Which of the following neither creates nor breaks any PDE bonds? (circle ALL that apply) a)telomerase b) topoisomerase/gyrase c) primase d) DNA pol 1 e) DNA poll 0) DNA ligase g) helicaseExplanation / Answer
Hi Answer:
1. Among the following which enzyme creates the MOST phosphodiester bonds during replication in E.coli?
Answer: Option C. cDNA polymerase III
DNA polymerase III is most productive enzyme and it creates about 500,000 phosphodiester bonds an average. Pol III is a multisubunit enzyme. It lacks a 5? to 3? exonucleolytic activity, although a subunit of the enzyme carries out the editing (3? to 5?) function during replication.
2. Which enzyme creates the FEWEST phosphodiester bonds during replication?
Answer: Option B. DNA polymerase I
Pol I is 5000 time less progressive than the pol II.
3. Which of the following can both create and break (hydrolyze) phosphodiester (PDE) bonds during replication?
Answer: Option B topoisomerase/gyrase
Topoisomerase consist a tyrosine residue, in this enzyme oxygen acts as a nucleophile which attacks the phosphate group in the phosphodiester bond and forms a covalent bond with it. This breaks the covalent bond to the next 5'nucleotide and leaves a free 3'-OH group on the upstream nucleotide. Whereas the topoisomerase allows free 3'-OH group to attack on the phosphate group. This activity reverses the tyrosine-phosphate linkage.
4. Which of the following necessarily creates as many PDE bonds as it breaks?
Answer: Option B topoisomerase/gyrase
5.Which of the following neither creates nor breaks and PDE bonds?
Answer: Option G helicase
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