3. Which method \'streak\' vs \"pour was most effective at getting isolated colo
ID: 272228 • Letter: 3
Question
3. Which method 'streak' vs "pour was most effective at getting isolated colonies and why? What are the advantages and disadvantages of each? 4. Explain how you could original liquid culture. 5. Classify each of the media used according to its functional type (ie. defined undefined, selective, differential). a. MSA: b. Blood Agar: c. TSA: d. M9 minimal media: How does MSA allow Staphylococci to grow and inhibits the growth of other microbes, and how do pathogenic forms of Staphylococci differ from non-pathogenic forms when grown on MSA? 6. Blood agar is used to determine if a species has the ability to do what? What does a positive result for this ability look like on blood agar? 7. Why might a microbiologist grow a bacterial species on a medium that contains the minimal nutrients necessary for its growth, such as M9 minimal media? 8. 9. How can you improve your aseptic technique?Explanation / Answer
Answer 3] Streak plate method is most effective at getting isolated colonies because
the successive streaks thin out the inoculum and is streaked across the surface of the agar medium.
Disadvantages of pour plate method=
1.The picking up of subsurface colonies needs digging them out of the agar medium thus interfering with other colonies.
2.The microbes being isolated must be able to withstand temporary exposure to the 42-45 0
temperature of the liquid agar medium:therefore this technique proves unsuitable for the isolation of psychrophilic microorganisms.
3.2.preparation for pour plate method is time consuming
Advantages of pour plate method=
1.also use for determining the number of viable bacterial cells present in a culture.
Advantages of streak plate method=
1. is used for the isolation into pure culture of the organisms,from mixed population.
2.to identify the organism
3.less time consuming
Disadvantages of streak plate method=
can not be used for enumeration of the approximate number of bacteria in a given sample.
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