I\'m confused on how in B, how the genotypes were identified, I know how to do t

Question

I'm confused on how in B, how the genotypes were identified, I know how to do the number of colonies but don't know how to match up the genotype with the numbers, or how to decide what the leading genotype is? why is man+ positive in all of the genotypes?

11. (12 points) Using P22 as a generalized transducing phage grown on a neo ser- man+ bacterial donor, a recipient strain of genotype neo sert man- is infected and incubated. Afterwards, 1000 man+transductants were selected. Note: sert is necessary to synthesize serine. All bacteria have the ability to utilize glucose as a sugar source. The mant is necessary for utilization of mannose.) a. What media was used for the man+ selection? Minimal Medium+mannose + serine (3 points) b. Replica plating is used to screen for the additional markers. The replica plates contain the following media (the presence of a supplement is indicated with a and its absence with a-). The number of colonies on each replica plate were shown at the bottom. (4 points) Media Replica 1 Replica 2 Replica 3 Replica4 eomycin Serine Mannose Glucose 1000 # colonies Fill out the table below with the genotypes of all possible transductants and the number of each type recovered 700 697 Genotvpe mant sertneoR man +ser -neoR man ser tneoS man ser -neoS # colonies 697 202 98

Explanation / Answer

Here they considered total colonies to 1000. Here they stated that the bacterial donor were infected the recipients and 1000 man+ transductants were selected. So all the genotypes contain man+. Based on the presence and absence of supplemented media components.

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